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TCMK-1 ATCC細胞購買

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  • 產品型號:ATCCCCL-139
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  • 更新時間:2019-04-17
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ATCCCCL-139 TCMK-1 ATCC細胞購買

基本信息
資源編號ATCCCCL-139
資源名稱TCMK-1
種屬Mus musculus, mouse kidney
類型SV40 transformed
形態epithelial
提供形式frozen
致病類型normal
安全等級2
培養方法
培養基The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
傳代方法Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
生長條件Temperature: 37°C
生長特性adherent
存儲條件liquid nitrogen vapor phase;Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
詳細說明
描述About 2% of the cells are positive for SV40 viral antigen, but virus is not recoverable.
Tested and found negative for ectromelia virus (mousepox).
Ageweanling
StrainC3H/Mai
Karyotypemodal number = 78; range = 64 to 157.All cells showed 2-6 acrocentric chromosomes with secondary constrictions and 1-4 biarmed chromosomes per cells. Sixty percent of the cells had one or more minute chromosomes.
DerivationThe cells are SV40 transformed and are positive for SV40 T-antigen.
Antigen ExpressionH-2k
TumorigenicNo
EffectsNo, in immunosuppressed mice
Yes, in semisolid medium
Virus SusceptibilityVesicular stomatitis, Glasgow (Indiana) Human poliovirus 1
Virus Resistancepoliovirus 1
Year of Origin1963

ATCCCCL-139 TCMK-1 ATCC細胞購買

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